Morphomolecular characterization of invasive fruit borer infesting apple

Apple, a vital fruit crop worldwide and a major crop grown in northern parts of India, acts as a backbone for the survival and livelihood of the farming community. However, it is prone to severe damage from insect pests and diseases. In the past few years, due to erratic weather patterns, there has been an alarmingly increased infestation of different insect pests, both invasive and non-invasive, resulting in substantial economic losses to this industry. One similar case was seen in the Himalayan region of India, where the apple crop in Karewas was heavily damaged by some invasive fruit borer, feeding on pulp and making galleries to reach the seed locules, thereby destroying the seed material. To ascertain the true identity of this pest, a study based on morpho-molecular characterization of this pest was carried out in the Faculty of Agriculture, Wadura, SKUAST Kashmir, India, during the years 2021 and 2022. The invasive fruit samples were collected from apple orchards at different locations (experimental sites) in North India by installing delta sticky traps @ 5 traps/ha for moth collection. The fruit-boring larvae and pupa were also collected and reared in confined chambers of the laboratory with controlled temperature and humidity. All the laboratory investigations were conducted at the Division of Entomology, FoA, Wadura, SKUAST Kashmir. During the investigation, it was confirmed that the invasive borer is a codling moth (Cydia pomonella L.), a member of the family Tortricidae, order Lepidoptera, which was earlier having quarantine importance in India, as it was only present in the Ladakh region of India. From the phylogenetic analysis of sample sequences, the species of codling moth present at all experimental sites was more identical to the codling moth sequence from Leh (the northernmost arid region), India. Further, the study of life cycle and voltinism revealed that codling moth completes three generations per year in Kashmir, with a single cycle lasting up to 2.5 months. However, the timing of various generations varies, depending on prevailing weather conditions at specific locations and times. The number of generations completed by codling moth in Kashmir conditions during apple growing season was three full and a half overwintering generations. The study of the life cycle of this important pest was necessary to know the weak link for its effective management and to prevent the economic loss in apple fruit.


Morphometric traits of fruit borer
The morphometric measurements of all the body parts revealed no significant difference in dimensions of the different body parts of invasive fruit borer samples collected from three locations.Head length and width ranged between 0.67-0.98mm and 1.01-1.27mm, respectively.The length of the antenna ranged from 4.43 to 5.59 mm.Proboscis length ranged between 1.01 and 2.06 mm.The length of labial palpi was in the range of 0.95-1.12mm.The morphometric dimensions of the head and its attached appendages are given in Table 1.The data on the length of the thorax, abdomen, forewing, hindwing, and frenulum is presented in Table 2.The thorax length was 3.00-3.98mm, however, the abdominal length ranged between 5.44-6.17mm.The forewing was somewhat longer in size than the hindwing, with lengths ranging between 7.35-8.31mm and 6.00-7.00mm, respectively.The length of the wing coupling apparatus, frenulum, was in the range of 1.19-1.42mm.The number of frenulum present per hindwing varied with sex, with males possessing only a single frenulum; however, more than one (2-3) per hindwing in the case of females.The morphometric measurements of different leg parts are shown in Table 3.The most significant part of the leg was the femur, and the smallest was the tarsal claw.The length of coxa, trochanter, femur, tibia, tarsus, and tarsal claw ranged between 1.03-1.14mm, 0.19-0.22mm, 1.39-1.50mm, 0.60-0.67mm, 1.33-1.40mm, and 0.08-0.09mm, respectively.The morphometric data of male and female genitalia of invasive fruit borer is presented in Table 4.The main parts of male genitalia (Fig. 6A) include a pair of valva and aedeagus.In contrast, in the case of female genitalia, the main parts are the corpus bursa, ductus bursa, signa, papillae anales, apophyses anteriores, and apophyses posteriores.The length of valva (Fig. 6B) and aedeagus (Fig. 6C,D) was 1.53-1.79mm and 0.85-0.97mm, respectively.In the case of female genitalia (Fig. 6E,F), the main parts include corpus bursae, ductus, papillae anales, apophyses posteriores, and apophyses anteriores, whose length ranged between 1.23-1.89mm, 0.39-0.67mm, 0.50-0.58mm, 0.45-0.56mm, and 0.98-1.06mm, respectively.This invasive borer had holometabolous development, possessing all 4 life cycle stages: egg, larva, pupa, and adult.The morphometry of different life stages is given in Table 5.The data revealed a non-significant difference in measuring different life stages of invasive fruit borer samples collected from the selected locations.The length of the adult, pupa, and larva and the diameter of the egg were found in the range of 9.93-11.05mm, 9.00-9.98mm, 10.89-12.94mm, and 0.89-1.19mm, respectively.

Molecular characterization
After running the BLASTn tool of NCBI, it was found that the query sequences of the current study (OQ186859, OQ190153, and OQ187770) showed maximum resemblance of 99.85 percent with two gene sequences, MW270024 and OQ836351 from Leh, India and Netherlands, respectively, belonging to codling moth species (Cydia pomonella L.).The results revealed that our query sequences had less intraspecific genetic distance of 0.0015 percent with sequence MW270024 from Leh, India, followed by OQ836351 from the Netherlands and GU095782 from Canada (0.0016% each) (Table 6).It was evident from the phylogenetic tree (Fig. 1) that the sequences separated to form two main clusters, and all three query sequences got their placement in the same www.nature.com/scientificreports/cluster along with sequence MW270024, which shows the invasive borer samples from three locations Nadihal, Chak Gojri and Delina belong to single species only and identical with the species of codling moth (Cydia pomonella L.), from Leh, India.Thus, morphometry, as well as molecular characterization, revealed that invasive fruit borer infesting apples in karewas of North Kashmir is a codling moth (Cydia pomonella L.) belonging to the family Tortricidae of order Lepidoptera.

Taxonomic description
The critical characteristics observed during our study revealed that the body is covered by dusky grey scales, which sometimes appear as small lenses.Two main mouth parts are tusk-shaped labial palpi and unscaled delicate coiled proboscis.A many-segmented long antenna has one row of scales on each segment.Males have an evident fascicle of long dark hairs in the cubital vein (Cu).The number of frenulum present per hindwing varies with sex, with males having only one and females having more than one (2-3).The male genitalia has a pair of valva, bearing straight costa with a round venter.There is a notch in the neck with a ventral spine on each valva, and the apical part of the valva is covered in dense hairs.Aedaegus has 7 spines-like structures in two groups: 2 at the center and 5 at the apex.Female genitalia contain round corpus bursae, with tiny thorn-like structures called signa, short ductus bursae, papillae anales, and apophyses anteriores longer than apophyses posteriores.

Feeding behavior
The staging stage of this pest is a larva, which enters the fruit usually through calyx and makes small galleries inside pulp to reach the seed locules and feed entirely on the seed material, particularly the endosperm.After reaching maturity, the fully grown larva emerges by making small exit holes covered with frass and excreta, which are then overwinter under loose bark or other suitable places.

The life cycle of Cydia pomonella L
Codling moth, just like other lepidopterans, possess four stages in its life cycle viz., egg, larva, pupa, and adult.The life cycle of codling moth, C. pomonella L. is shown in Fig. 2, and the duration of different life stages is given in Table 7.It was evident from laboratory rearing experiments of codling moths that females laid eggs singly on the fruits, and few eggs were laid on leaves provided inside the rearing cages.The eggs (Fig. 7A) were somewhat discoid, resembling a convex lens and having a diameter in the 0.89-1.19mm range.Freshly laid eggs were translucent and milk white; however, they changed color to reddish brown while approaching hatching, showing www.nature.com/scientificreports/ a black spot indicating the head of a 1st instar larva.The average duration of egg up to hatching was about 8-10 days.The larval stage is the only damaging stage of codling moth.The color variation was quite visible, as the 1st instar larva, just after hatching, was creamy white with a brown head wider than the thoracic segments, while as the mature 5th instar larva, after exiting from fruit, was pinkish red or pinkish brown with a blackish brown head.After hatching the egg, 1st instar larva usually enters the freshly placed apple fruits through the calyx end.It makes minute galleries by feeding on the pulp to reach their ultimate destination (seed locules).The larva ultimately feeds on the seed material and comes out by making exit holes covered with frass and excreta for overwintering.The average length of mature larva (Fig. 7B) was 10.89-12.94mm, with a mean duration of 21-30 days from hatching to overwintering.After exiting the fruit, the mature 5th instar larva searches for the overwintering site, for which it enters the already placed small cardboard strips and spins a white cottony cocoon inside the galleries in cardboard strips.After some period, the overwintering larva begins to form a brown-colored pupa-its color changes from light brown to dark brown as it advances towards adult emergence.There was a difference in the length of the pupa as per its sex; the female pupa was somewhat longer than the male pupa.However, the length of the pupa (Fig. 7C) was in the range of 9.00-9.98mm.The pupal stage was about 12-14 days, and after that, the adult came out by breaking the pupa from the head end.After emerging from the pupa, the adults appeared similar, covered by dusky grey scales.There were distinct dark lines on the extreme portion of the forewings.The adult females laid eggs singly on freshly placed fruits and leaves.The average adult length (Fig. 7D) was 9.93-11.05mm, and the adult duration was 14-16 days.

Number of generations
The codling moth completed three generations in Kashmir throughout the apple growing season.In addition to three generations, half of the overwintering larva was observed at the experimental site as temperatures dropped to minus during harsh winter.There was a difference in the onset of first-generation moths and the end of the last generation for the consecutive study years, and the comparison is shown in Table 8.During 2021, the first generation of codling moths commenced from the 18th standard meteorological week (SMW) and ended in the 26th SMW; the second generation began in the 27th SMW and ended in the 34th SMW.However, the third generation started in the 35th SMW and ended in the 43rd SMW.In contrast, during 2022, the first generation of codling moth commenced from 16th SMW and ended in 25th SMW, and the second generation commenced from 26th SMW and ended in 34th SMW; however, the commencement and end of the third generation were in 35th and 42nd SMW, respectively.The perusal of data on the commencement of the first generation and end of the third generation of the codling moth for 2021 and 2022 revealed an early emergence of the first generation and early completion of the last generation in 2022 compared to 2021.

Discussion
In the present investigation, the morphometry of all possible body parts of invasive fruit borer was studied.However, literature was not available on the characters, such as the head and its appendages, thorax, and leg, and this type of detailed work on morphometry of codling moth was probably done for the first time.Our results for   www.nature.com/scientificreports/wing dimensions were in close conformity with the reports of Pinhey 21 , who measured the length of the forewing 7-8 mm with a striped appearance with the apical half of the wing medium brown.The number of frenulum per hindwing helped know the sex of invasive fruit borer samples, as males possessed a single frenulum, while females more than one (2-3), and this was in line with the findings of Common 22 , who also reported a single unbranched frenulum in male codling moths, while females possessing more than one (2-6).The size of different life stages of fruit borer was in agreement with that of Bonnemaison 23 , who recorded the size of an egg, larva, and pupal stages as 1 mm, 19 mm, and 1 cm, respectively.Similarly, MacKay 24 reported the length of the larval stage of codling moth within the range of 15-19 mm.However, Brown 25 reported the same in the 14-18 mm range.Another report of codling moth females laying eggs of 1 mm diameter and larva transforming into pupal stage of average length as 9.64 mm 26 .Similarly, one more study supported our statement that the size of full-grown last instar larvae of codling moth was 11.98 mm 27 .DNA barcoding based on mtCOX1 gene sequences (OQ186859, OQ190153, and OQ187770) of invasive borer samples revealed that species at all three locations were identical with each other and showed maximum resemblance of 99.85 percent with two sequences MW270024 28 and OQ83635 29 belonging to codling moth, Cydia pomonella L. from Leh, India and Netherlands, respectively.The least intra-specific distance of query sequences with the sequences mentioned above and a phylogenetic tree made the identity more apparent, as the former shared the same clade with sequence MW270024 28 , which shows the invasive borer samples from selected locations Nadihal, Chak Gojri, and Delina belong to same species, Cydia pomonella L. and further these were similar to the codling moth from Leh, India 28 .Therefore, it is evident that the pest that created massive damage in apple orchards of North Kashmir was a codling moth, which previously was of quarantine importance, as it was restricted only to Ladakh, India 14 .
The codling moth, C. pomonella L. is a pest of almost all pome fruits belonging to the family Tortricidae of order Lepidoptera.The moth's body is covered by dusky grey scales, sometimes appearing as small lenses.Our results closely conform with those of Hussain et al. 26 , who reported codling moths with greyish brown color and coppery wings with dark-colored tips.Mouth parts include a pair of tusk-shaped labial palpi and unscaled fine-coiled proboscis.Rentel 30 also reported that the head bears tusk-shaped labial palpi and smooth proboscis.A many-segmented long antenna has a row of scales on each segment.Males have an evident fascicle of long dark hairs in the cubital vein (Cu).Genitalia, an important character to study while identifying a pest, is also vital here.The description given about genitalia is in agreement with findings of Rentel 30 , who reported the presence of a pair of valva with round venter and straight costa, densely covered with hairs and an aedeagus bearing 7 spines-like structures in the male genitalia of codling moth, the female genitalia containing corpus bursae with two signa on surface and ductus bursae 24 .The damaging stage of this pest is a larva, which enters the fruit usually through calyx and makes small galleries inside pulp to reach the seed locules and feed entirely on the seed material, particularly the endosperm.Hussain et al. 26 and Ju et al. 31 validate our finding by mentioning the codling moth as a direct pest whose larvae bore into the fruits, feed on seeds, and create exit holes by pushing waste material out.At maturity, the fully grown larva emerges by making small exit holes covered with frass and excreta, which then overwinter under loose bark or other suitable places.Similar results were obtained by Pajač et al. 15 , who stated that the pest overwinters as completely grown larvae within a thick, silken cocoon that can be found under loose scales of bark and in soil or debris around the tree base, where it pupates after that in early spring.Our results reported four stages in the life of the codling moth, C. pomonella L. viz., egg, larva, pupa, and adult, with a duration of 8-10, 21-30, 12-14, and 14-16 days, respectively.Similar results were recorded on the biology of codling moths by Pajač et al. 15 , who reported the completion of egg, larval, and pupal stages of codling moths in 5-12 days, four weeks, and 7-30 days, respectively.In the same way, another researcher recorded the duration of the egg as 6-14 days while that of the pupal stage as 10-20 days 3 .Similarly, adult longevity was recorded by Kuyulu and Genc 32 separately in female and male sex on 10% sugar solution diet as 12.20 ± 0.60 days and 16.70 ± 1.90 days, respectively.
It was seen that codling moth females lay eggs singly on the fruits and sometimes leaves.The eggs were somewhat discoid, resembling a convex lens and having a diameter of 0.89-1.19mm.Freshly laid eggs were translucent and milky white.However, there was a change in color to reddish brown while approaching hatching, showing a black spot that indicates the head of the 1st instar larva.The findings related to the shape and size of the egg stage were in accord with the findings of Bonnemaison 23 , who found the eggs of codling moth flat and discoid, having a diameter of 1 mm, white and later on showing a black head with red ring towards hatching.The fecundity and site of oviposition were confirmed by Pajač et al. 15 , who found that a female lays 30-70 eggs singly on the upper surface of leaves or fruit surface.Similar findings were recorded by other researchers too at different times and places 3,[33][34][35][36][37] .Our study revealed that the larval stage is the only damaging stage in the codling moth.This aligns with the findings of Powell 38 and Timm 39 , who reported the larval stage of most tortricids, including codling moth, as a major threat to the fruit industry.The color variation was quite visible, as the 1st instar larva, just after hatching, was creamy white with a brown head wider than the thoracic segments.
In contrast, after exiting from fruit, the mature 5th instar larva was pinkish red or pinkish brown with a blackish brown head.The average length of mature larvae was in the range of 10.89-12.94mm.Our results are in parallel with the findings of Bonnemaison 23 , who mentioned the color of larva as creamy white with a black head initially, which eventually turns to pink towards maturity, and the size of a full-grown larva as around 19 mm long.Similarly, Hussain et al. 3 reported that the 5th instar larva of codling moth is pink and has a brownish head.The feeding behavior observed in this pest was in agreement with Kuhrt et al. 40 , who reported that codling moth larvae feed on the pulp of fruit by making large cavities at the pericarp.The results of Kuyulu and Genc 32 support our findings regarding the overwintering of codling moths in the late larval stage and the difference in the length of pupa concerning sex; female pupa was somewhat longer than male pupa, with an average size of 9.62 mm.At the same time, results regarding adult description are also supported by Kuyulu and Genc 32 .The number of generations completed by codling moth in Kashmir during apple growing season was three full and a half overwintering generations.The data is supported by findings of Borden 33 , Jackson 34 and Geier 35 , who quoted in their study that codling moth can complete 1-3 generations annually depending on the environmental conditions and longevity of the growing season in a particular area.Similarly, the findings of Chapman 41 and Audemard 42 agree with our results.They have mentioned that codling moth can complete only single generation in colder areas while 4-5 generations in warm and hot weather conditions.However, the time of onset and end of the first and last generation for both study years was different.This could be because weather variables directly influence pest population dynamics, and in certain areas, the growing season is of short duration, while in other areas, it is quite long.In 2022, the spring months, particularly March, had more hot weather than 2021, which caused the early emergence of codling moths in 2022 than 2021.Also, there was early ripening and harvesting of apples during 2022 due to an overall rise in temperature for the entire growing season compared to 2021, which caused early completion of the life cycle of the last generation moth.All these factors tremendously impact the activity and emergence of different pests, including codling moths.Hussain et al. 14 got the first trap catch of adult males of codling moth on 21st May while working in Ladakh, India, which is having a short growing season due to harsh cold climatic conditions.However, in apple orchards of Northern Kyrgyzstan, having hot and humid weather conditions, Tair and Levent 43 got their first male trap catch of codling moth in April.

Conclusions
The morphomolecular characterization of invasive fruit borer in apples under Kashmir conditions revealed that the pest responsible for premature fruit drop and huge yield loss was the codling moth (Cydia pomonella L.), belonging to the family Tortricidae of order Lepidoptera.The possible route of its first invasion in Kashmir was from Ladakh, as the phylogenetic analysis of gene sequences (OQ186859, OQ190153, and OQ187770) of this pest from all three experimental locations resembled the codling moth sequence from Leh Ladakh (MW270024).Further, the life cycle study revealed that it completes three full and a half overwintering generations under Kashmir climatic conditions with a duration of the single cycle as 2.5 months.The life cycle study was essential to know the weak link of this pest for its effective management and to prevent economic loss in Apple.

Collection of borer sample
The invasive fruit borer samples (adult moths, pupae, and larvae) were collected from apple orchards at three locations in North Kashmir, India viz., Nadihal (Latitude 34°15′34″N; Longitude 74°21′48″E and 1678 m amsl), Delina (Latitude 34°14′26″N; Longitude 74°24′58″E and 1606 m amsl)and Chak Gojri (Latitude 34°16′47″N; Longitude 74°22′42″E and 1618 m amsl), (Fig. 3A).At the experimental site, delta sticky traps (Fig. 3B) procured from the Division of Entomology, Faculty of Horticulture, Shalimar, SKUAST Kashmir were installed @ 5 traps/ ha for the collection of moths (Fig. 3C).These sticky traps were equipped with a lure containing female pheromone to entrap male moths.These delta traps were kept intact at its primary place unless damaged; however, the sticky white liners were replaced fortnightly with fresh ones if needed, to ensure the effective trapping of male moths.During the whole study period, 42 traps and 118 liners were used.Simultaneously, fruit boring larvae and pupae were collected from experimental sites for rearing in a laboratory.All the laboratory investigations were carried out at the Division of Entomology, FoA Wadura (Latitude 34°20′54″N; Longitude 74°24′02″E and 1606 m amsl), SKUAST Kashmir, between 2021 and 2022.The rearing procedures followed were based on the study done by Kuyulu and Genc 32 , and specific climatic conditions were maintained under confined chambers in the laboratory, with temperature 25 ± 1 °C, relative humidity 60 ± 5%, and 16:8 (light: dark) hour photoperiod.

Microscopy and morphometry of samples
The specimens of invasive fruit borer moths collected from experimental sites, as well as those reared in the laboratory as per the procedures followed by Dickson et al. 44 , Geier and Briese 45 , Vetter et al. 46 and Kuyulu and Genc 32 , were prepared for microscopy.The live moths were placed in killing jars and anesthetized with ethyl acetate.Wings and legs were detached first, followed by the dissection of the general body using fine needles, forceps, and small sharp blades.The male and female genitalia were drawn out from the abdomen very carefully with the help of fine brushes.Male genitalia were obtained without any abdominal part attached to it.However, female genitalia were drawn out along with the 7th sternite, as it is often fused with the sterigma.The dissected body parts of adult moths were then prepared for microscopy following Horak 47 , Robinson 48 , Park 49 , Common 50 , Zimmerman and Klots 51 procedures.After keeping the dissected parts overnight in 10 (%) KOH solution (Fig. 3D) for relaxation of the musculature, the specimens were thoroughly washed with distilled water 2-3 times and then immersed in 70 (%) ethanol (Fig. 3E) for about 1 h.This was followed by the overnight placement of specimens in a clearing agent (xylene and phenol in a 3:1 ratio) (Fig. 3F).The scales were removed with the help of a fine camel hair brush.Some body parts, completely transparent and hard to describe under such conditions, were stained by a dye prepared by mixing 1gm of acid fuchsin in 100 ml of 50% ethanol with 5 ml glacial acetic acid.After cleaning, dehydrating, and flattening of genitalia and other body parts, they were placed and mounted on clean, dry standard microscopic slides in Canada Balsam and covered with a coverslip for microscopy and morphometry.A stereo zoom microscope with Microview software calibrated with a fixed known ruler, the stage micrometer, was used to measure minute structures.However, other body parts of large size, like the abdomen, thorax, and wings, were measured with the help of a normal measuring scale.All the dissected parts were photographed directly using the same stereo zoom microscope, attached with a digital camera for authentic pest identification.

Morphometric traits of fruit borer studied
The general body parts analyzed for morphometry include the head, thorax, abdomen, appendages, and male and female genitalia.The length and width of the head (Fig. 4A) were measured from the anterior-most of the body to the origin of mouth appendages and from one compound eye to the other, respectively.The antennal length (Fig. 4B) was measured right from the scape to the end of the flagellum.As the fruit borer had completely coiled proboscis (Fig. 4C), it was somehow unwinded and flattened to measure its length.Measurements of two important mouth parts, labial palpi and reduced maxillary palpi (Fig. 4D), were also taken.The dimension of the thorax (Fig. 5A) was taken right from the detachment point of the head to the beginning of the first abdominal segment.In contrast, the abdominal length (Fig. 5B) was calculated from the end of the thorax to the posterior end of the moth body.The length of the forewing (Fig. 5C) and hindwing (Fig. 5D) was calculated from the humeral angle to the apex.The size of the frenulum and the number of frenuli (Fig. 5E) present per wing concerning sex were also measured.The length of the leg (Fig. 5F) was taken by analyzing different parts viz., coxa, trochanter, femur, tibia, tarsus, and tarsal claw.Various parts of male and female genitalia (Fig. 6) after processing were measured with the help of microview software and a stage micrometer.In addition, all life cycle stages were measured (Fig. 7).

Life cycle assessment
For the study of the life cycle and number of generations completed per year by codling moth, fruits of the Red Delicious apple variety infested with fruit borer (Fig. 8A) were collected from the experimental site (Nadihal) and brought to the laboratory of Division of Entomology, Faculty of Agriculture, Wadura, SKUAST-K.In addition, the overwintering stages and pupa were also collected and brought to the laboratory to create the initial culture (Fig. 8B) of the invasive fruit borer.The infested fruits were placed in plastic/ glass containers along with pieces of corrugated cardboard strips as pupation sites (Fig. 8C), and the open end of the container was covered with muslin cloth tied with the help of rubber bands.These containers were monitored daily in the laboratory for various developmental stages of the pest.Adults emerging from their respective pupae (Fig. 8D) were transferred to other containers inside the rearing cages and fed with a 10% honey/sugar solution (Fig. 8E), facilitated with fresh fruits and twigs for oviposition.Eggs laid by female moths (Fig. 8F) were observed daily until the emergence of larva and were then facilitated with fresh apple fruits to penetrate and feed on them.After the entry of larvae in the fruits through the calyx, infested fruits were observed keenly for the larvae exit, and corrugated cardboard strips were placed in the same container to facilitate pupation.Pupae and corrugated cardboard strips were placed in separate containers and keenly supervised until the adult emergence.After this, adults were again given a 10 percent honey/sugar solution as food soaked on cotton balls.The whole rearing process is shown in Fig. 8.The time taken for transforming one stage into another developmental stage was noted, and the time taken to complete the life cycle by fruit borer and the number of generations completed were calculated accordingly.

Statistical analysis
The data obtained was subjected to standard statistical analysis using R software, and significant results of different body parts among the three experimental locations were compared based on critical differences.The data on morphometry of various body parts of codling moth was expressed as mean ± SE values, and the range was also calculated for morphometric data across all the locations.

Molecular characterization
The samples of invasive fruit borer were sent to ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru, where DNA barcoding/molecular characterization and taxonomic description were carried out in the Division of Genomics.An outsourced agency, Eurofin Genomics, did the sequencing of PCR products.After obtaining the mitochondrial COX1 sequences of samples of invasive fruit borer from all three locations, the sequences were submitted to the National Centre for Biotechnology Information (NCBI), and with the help of the BLASTn tool, further identification of invasive fruit borer was made.A phylogenetic tree was constructed to evaluate evolutionary similarities, and intra-specific distance was also calculated in MEGA 11 software, busing the barcodes of all three sample sequences.The accession numbers are given in Table 9.The intra-specific distance between three query sequences and seven sequences from GenBank was calculated.Table 9. Accession numbers and barcode details of invasive fruit borer samples sent to NBAIR for identification.

Figure 1 .
Figure 1.Phylogenetic tree (Neighbor-joining) depicting evolutionary similarities between the query sequences and sequences retrieved from GenBank.

Figure 3 .
Figure 3. (A) Satellite map of experimental sites, (B) installation of delta traps, (C) trap catch of adult moths and (D-F) sample preparation for microscopy.

Table 1 .
Morphometrics of head, antenna, proboscis, and labial palpi of Cydia pomonella L. *Mean of 10 replications.a Means bearing the same letter within the same column of the same parameter are not significantly different at p < 0.01.

Table 3 .
Morphometrics of the leg of Cydia pomonella L. *Mean of 10 replications.a Means bearing the same letter within the same column of the same parameter are not significantly different at p < 0.01

Table 4 .
Morphometrics of male and female genitalia of Cydia pomonella L. *Mean of 10 replications.a Means bearing the same letter within the same column of the same part of parameter are not significantly different at p < 0.01.

Table 5 .
Morphometrics of different stages in the life cycle of Cydia pomonella L. *Mean of 10 replications.Means bearing the same letter within the same column of the same parameter are not significantly different at P < 0.01.

Table 7 .
Duration of different stages in life cycle of Cydia pomonella L.

Table 8 .
Variation in the commencement and end of three generations of Cydia pomonella L. during consecutive study years.